| 货号 | 4194S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F |
| 目标/特异性 | Sox1 Antibody detects endogenous levels of total Sox1 protein. It does not cross-react with endogenous levels of other Sox proteins, including Sox2 and Sox3. |
| 使用方法 | WB(1:1000) IF-F (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sox (Sry-related box) genes encode a family of transcription factors that play a myriad of roles in developmental processes (1). Sox1 and Sox2 are the earliest markers that identify neuroectodermal tissue, and these markers, together with Sox9 collectively mark neuronal stem cells (NSCs) that are present in neurogenically active areas of the mature rodent brain (2). Sox1 has been found in the walls of the lateral ventricles and the dentate gyrus, as well as in inactive areas such as the cerebellum where it marks a population of cells in the Purkinje layer known as the Bergmann glia (2). Sox1 is thought to maintain the cell cycle and promote self renewal in NSCs, but also functions in other cell types to promote differentiation (3). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Kiefer, J.C. (2007) Dev Dyn 236, 2356-66. 2 . Alcock, J. et al. (2009) Neurosci Lett 450, 114-6. 3 . Kan, L. et al. (2007) Dev Biol 310, 85-98. |
Confocal immunofluorescent analysis of postnatal day 1 (left) and adult (right) rat brain using SOX1 Antibody (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用SOX1抗体（绿色）和Neurofilament-L (DA2) Mouse mAb #2835 （红色）对出生后1天（左）和成年（右）大鼠脑组织进行激光共聚焦荧光分析。蓝色假色=DRAQ5® #4084 (DNA 荧光染料). | |
Western blot analysis of extracts of fetal rat brain, mouse brain and E6-5 embryo, using Sox1 Antibody.使用Sox1抗体对胚胎期大鼠脑、小鼠的脑和E6-5胚胎提取物进行western blot分析。 |
