| 货号 | 4973S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | SnoN Antibody detects endogenous levels of total SnoN protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis and disease pathogenesis (1-3). Upon stimulation by TGF-β, activated receptors phosphorylate Smad2 and Smad3, resulting in their translocation to the nucleus, association with Smad4 and transcriptional regulation of target genes (4). Ski and SnoN are related oncoproteins originally discovered based on homology to v-Ski, the transforming protein of the Sloan-Kettering virus (5). They regulate TGF-β signaling by binding to Smad2 and Smad4 and repressing their ability to activate transcription (6). Following TGF-β stimulation, SnoN is rapidly degraded by the ubiquitin proteasome pathway providing negative feedback regulation (6-9). Overexpression of SnoN and Ski can transform avian fibroblasts and induce muscle differentiation (10). Mice heterozygous for SnoN and Ski display increased susceptibility to tumorigenesis (11,12). Interestingly, elevated expression of Ski and SnoN has been observed in many tumors and may serve as important prognostic markers (13,14). Taken together, these studies suggest possible dual functions of these proteins at different stages of tumorigenesis (15). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Massague, J. et al. (2000) Cell 103, 295-309. 2 . Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402. 3 . Derynck, R. et al. (2001) Nature Genet. 29, 117-129. 4 . Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157. 5 . Nomura, N. et al. (1989) Nucleic Acids Res. 17, 5489-5500. 6 . Stroschein, S.L. et al. (1999) Science 286, 771-774. 7 . Bonni, S. et al. (2001) Nat. Cell Biol. 3, 587-595. 8 . Stroschein, S.L. et al. (2001) Genes Dev. 15, 2822-2836. 9 . Wan, Y. et al. (2001) Mol. Cell 8, 1027-1039. 10 . Boyer, P.L. et al. (1993) Oncogene 8, 457-466. 11 . Shinagawa, T. et al. (2001) Oncogene 20, 8100-8108. 12 . Shinagawa, T. et al. (2000) EMBO J. 19, 2280-2291. 13 . Zhang, F. et al. (2003) Cancer Res. 63, 5005-5010. 14 . Buess, M. et al. (2004) Neoplasia 6, 207-212. 15 . Zhu, Q. et al. (2007) Mol. Cell. Biol. 27, 324-339. |
Western blot analysis of extracts from SW620, HT-1080 and MCF-7 cells using SnoN Antibody.使用SnoN抗体对620, HT-1080 和 MCF-7 细胞提取物进行Western blot分析。 | |
Immunofluorescent analysis of A-204 cells showing nuclear staining, using SnoN Antibody.使用SnoN抗体对 A-204 细胞进行免疫荧光分析，显示细胞核染色。 |
