| 货号 | 3757S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SNIP/p140Cap Antibody detects endogenous levels of total SNIP/p140Cap protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SNIP (SNAP25-interacting protein)/p140Cap (p130Cas-associated protein) is a cytoskeleton-associated protein identified initially in rat as a protein interacting with the brain-specific synaptosome protein SNAP25 (1) and subsequently as interacting with the broadly expressed scaffold protein p130Cas (2). SNAP25, a presynaptic protein implicated in neurotransmitter secretion, membrane fusion and neurite outgrowth, is part of the SNARE complex that includes syntaxin and synaptobrevin/VAMP (3). SNIP-SNAP25 association is mediated by coiled-coil interactions (1). Overexpression of SNIP inhibits calcium-dependent exocytosis in PC12 cells (1). Human and mouse orthologs of SNIP, termed p140Cap, were subsequently identified through association with p130Cas, a substrate of v-Src and v-Crk that is tyrosine-phosphorylated in response to cell adhesion and mitogenic stimuli (2,4,5). Expression of p140Cap was observed in brain, testis and epithelial-rich tissues and may exist in various alternatively spliced, tissue-specific isoforms (2). p140Cap is also tyrosine-phosphoryalated in response to adhesion molecules and EGF treatment (2). Together these studies suggest a role for SNIP/p140Cap in controlling cell spreading, migration and neurosecretion. |
| 存放说明 | -20C |
| 计算分子量 | 145 |
| 参考文献 | 1 . Chin, L.S. et al. (2000) J. Biol. Chem. 275, 1191-1200. 2 . Di Stefano, P. et al. (2004) Mol. Biol. Cell 15, 787-800. 3 . Gerst, J.E. (1999) Cell. Mol. Life Sci. 55, 707-734. 4 . Sakai, R. et al. (1994) EMBO J. 13, 3748-3756. 5 . Defilippi, P. et al. (2006) Trends Cell Biol. 16, 257-263. |
Western blot analysis of extracts from K562, SH-SY5Y, IMR32, Neuro2A and PC12 cell lines using SNIP/p140Cap Antibody. 对K562、SH-SY5Y、IMR32、Neuro2A和PC12细胞系裂解液使用SNIP/p140Cap Antibod进行Western blot分析。 |
