| 货号 | 4251S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SMYD2 Antibody detects endogenous levels of total SMYD2 protein. The antibody does not cross-react with SMYD3 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), is a member of the SMYD family of protein methyltransferases (1). All five members of this family (SMYD1, SMYD2, SMYD3, SMYD4, and SMYD5) contain a conserved catalytic SET domain, originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins. This domain is split by the MYN domain/zinc finger motif believed to facilitate protein-protein interactions (1). SMYD2 localizes to both the cytoplasm and nucleus, and is highly expressed in the adult mouse heart, brain, liver, kidney, thymus, and ovary, as well as in the developing mouse embryo (1). SMYD2 functions to repress transcription by interacting with the Sin3A repressor complex and methylating Lys36 of histone H3 (1). SMYD2 also interacts with HSP90α and methylates Lys4 of histone H3, a mark associated with transcriptional activation (2). In addition to histones as methyl substrates, SMYD2 methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
| 参考文献 | 1 . Brown, M.A. et al. (2006) Mol Cancer 5, 26. 2 . Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72. 3 . Huang, J. et al. (2006) Nature 444, 629-32. 4 . Huang, J. et al. (2007) Nature 449, 105-8. |
Western blot analysis of extracts from various cell lines using SMYD2 Antibody. 使用SMYD2 Antibody，免疫印迹(Western blot)分析不同细胞中SMYD2的蛋白水平。 |
