| 货号 | 9592S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | SMG-1 (V72) Antibody detects endogenous levels of total SMG-1 protein. The antibody cross-reacts with a 100 kDa band of unknown origin. By confocal immunofluorescence, the antibody labels the mitochondria of U-2 OS cells (7). |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SMG-1 is a member of the phosphoinositide 3-kinase-related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA-PKcs, and TRRAP (1,2). Activated by DNA damage, SMG-1 has been shown to phosphorylate p53 and hUpf1 (SMG-2) (1-4). hUpf1 is a subunit of the surveillance complex that allows degradation of messenger RNA species containing premature termination codons (PTCs). This process, known as nonsense-mediated mRNA decay (NMD), prevents the translation of truncated forms of proteins that may result in gain of function or dominant negative species. NMD occurs under normal cellular conditions as well as in response to damage (5,6). SMG-1 has also been shown to affect cell death receptor signaling and to protect cells from extrinsically induced apoptotic cell death (7).SMG-1是磷酸肌醇3激酶相关激酶(PIKK)家族的一员，该家族包括ATM，ATR，mTOR，DNA-PKcs和TRRAP (1,2)。经由DNA损伤激活，SMG-1已经被发现能够磷酸化p53 和hUpf1 (SMG-2) (1-4)。hUpf1是监视复合体的一个亚基，该复合体允许信使RNA物种(包含提前终止密码子PTC)的降解。该过程亦被称作无意义介导的mRNA降解(NMD)，可以阻止蛋白截断体形式的翻译，也能够引起功能获得或者优势阴性物种。NMD在正常细胞环境中发生，同时也应答DNA损伤(5,6)。SMG-1亦被认为能够影响细胞死亡受体信号和保护细胞外部诱导凋亡性细胞死亡(7)。 |
| 存放说明 | -20C |
| 计算分子量 | 410 |
| 参考文献 | 1 . Denning, G. et al. (2001) J Biol Chem 276, 22709-14. 2 . Yamashita, A. et al. (2001) Genes Dev 15, 2215-28. 3 . Brumbaugh, K.M. et al. (2004) Mol Cell 14, 585-98. 4 . Ohnishi, T. et al. (2003) Mol Cell 12, 1187-200. 5 . Li, Z.Y. et al. (2006) Curr Med Chem 13, 1693-705. 6 . Mendell, J.T. et al. (2004) Nat Genet 36, 1073-8. 7 . Oliveira, V. et al. (2008) J Biol Chem 283, 13174-84. |
Confocal immunofluorescent analysis of U-2 OS cells using SMG-1 (V72) Antibody (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos #9082 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HT-29, COS and U-2 OS cells using SMG-1 (V72) Antibody.Western blot方法检测HT-29, COS和U-2 OS细胞提取物，使用的抗体为SMG-1 (V72) Antibody。 |
