| 货号 | 4802S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SMC1 Antibody detects endogenous levels of total SMC1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).染色体结构维持蛋白1(SMC1)是黏连蛋白复合体的一个染色体蛋白成员，该复合体促进姐妹染色单体结合并在DNA修复中起着重要作用(1,2)。作为S期内部DNA损伤检验点的一部分，ATM/NBS1-依赖的SMC1磷酸化发生在第957位和966位丝氨酸以应答离子辐射(IR) (3)。在经历其他形式的DNA损伤包括紫外线和羟基脲处理的细胞中，SMC1的磷酸化是ATM-非依赖的(4)。尽管SMC1的磷酸化是IR诱导的内S期检验点激活所必需的，但精确的机制尚未阐明且有可能涉及影响SMC1-SMC3相互作用的构象变化(3)。 |
| 存放说明 | -20C |
| 计算分子量 | 145 |
| 参考文献 | 1 . Michaelis, C. et al. (1997) Cell 91, 35-45. 2 . Sjögren, C. and Nasmyth, K. (2001) Curr Biol 11, 991-5. 3 . Yazdi, P.T. et al. (2002) Genes Dev 16, 571-82. 4 . Kim, S.T. et al. (2002) Genes Dev 16, 560-70. |
Western blot analysis of extracts from 293 cells, untreated or UV-treated, using Phospho-SMC1 (Ser957) Antibody #4801 (upper) or SMC1 Antibody (lower).Western blot方法检测293细胞提取物。未处理或者紫外处理，使用的抗体为Phospho-SMC1 (Ser957) Antibody #4801 (上)或SMC1 Antibody (下)。 | |
Flow cytometric analysis of HeLa cells, using SMC1 antibody (blue) compared to a nonspecific negative control antibody (red).流式细胞术检测HeLa 细胞，使用 SMC1 抗体(蓝色)，相比较于非特异性阴性对照抗体(红色)。 |
