| 货号 | 8829S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SMARCC2/BAF170 Antibody recognizes endogenous levels of total SMARCC2/BAF170 protein (both isoforms 1 and 2). |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors such as nuclear receptors, p53, Rb, BRCA1, and MyoD facilitate recruitment of the complex to target genes to regulate gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). |
| 存放说明 | -20C |
| 计算分子量 | 162, 170 |
| 参考文献 | 1 . Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84. 2 . Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73. 3 . Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11. 4 . Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81. 5 . Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17. 6 . Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32. 7 . Morettini, S. et al. (2008) Front Biosci 13, 5522-32. 8 . Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6. 9 . Simone, C. (2006) J Cell Physiol 207, 309-14. 10 . Phelan, M.L. et al. (1999) Mol Cell 3, 247-53. 11 . Ho, L. et al. (2009) Proc Natl Acad Sci U S A 106, 5181-6. |
Western blot analysis of extracts from various cell lines using SMARCC2/BAF170 Antibody. 使用SMARCC2/BAF170 Antibody，免疫印迹(Western blot)分析不同细胞中SMARCC2/BAF170蛋白水平。 |
