| 货号 | 12458S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SMARCAD1 Antibody recognizes endogenous levels of total SMARCAD1 protein. This antibody also cross-reacts with a protein of unknown origin at 40 kDa. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SMARCAD1 is a SWI/SNF-like chromatin remodeling protein that plays a critical role in the maintenance of heterochromatin domains following DNA replication and proper chromosome segregation during mitosis (1-3). SMARCAD1 can be found in association with transcription repressor KAP1, histone deacetylases HDAC1/2, and lysine methyltransferase G9a/GLP, which are recruited to sites of DNA replication by PCNA. These proteins facilitate deacetylation of histones and methylation of histone H3 Lys9 to re-establish heterochromatin domains, such as centromeric regions (1). SMARCAD1 also plays a role in double stranded DNA break repair by facilitating DNA end resection and the subsequent repair by homologous recombination (4). Loss of SMARCAD1 results in increased sensitivity to DNA damaging agents, suggesting a role in the maintenance of genome stability. |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Rowbotham, S.P. et al. (2011) Mol Cell 42, 285-96. 2 . Mermoud, J.E. et al. (2011) Cell Cycle 10, 4017-25. 3 . Jasencakova, Z. and Groth, A. (2011) Mol Cell 42, 267-9. 4 . Costelloe, T. et al. (2012) Nature 489, 581-4. |
Western blot analysis of extracts from various cell lines using SMARCAD1 Antibody.Western blot方法检测多种细胞系的提取物，使用的抗体为 SMARCAD1 Antibody。 |
