| 货号 | 9450S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SMARCA1 Antibody recognizes endogenous levels of total SMARCA1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SMARCA1 (SNF2L) is one of the two orthologs of the ISWI (imitation switch) ATPases encoded by the mammalian genome (1). The ISWI chromatin remodeling complexes were first identified in Drosophila and have been shown to remodel and alter nucleosome spacing in vitro (2). SMARCA1 is the catalytic subunit of the NURF (nucleosome remodeling factor) and CERF (CECR2-containing remodeling factor) complexes (3-5). The NURF complex plays an important role in neuronal physiology by promoting neurite outgrowth and regulation of Engrailed homeotic genes that are involved in neuronal development in the mid-hindbrain (3). NURF is also thought to be involved in the maturation of T cells from thymocytes by regulating chromatin structure and expression of genes important for T cell development (6). The largest subunit of the NURF complex, BPTF, is required for proper development of mesoderm, endoderm, and ectoderm tissue lineages, suggesting a role for SMARCA1 in the development of the germ layers in mouse embryo (7). Disruption of the CERF complex by deletion of CECR2, an interacting partner of SMARCA1, is associated with the neural tube defect exencephaly, linking the CERF complex with regulation of neurulation (4). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Lazzaro, M.A. and Picketts, D.J. (2001) J Neurochem 77, 1145-56. 2 . Erdel, F. and Rippe, K. (2011) FEBS J 278, 3608-18. 3 . Barak, O. et al. (2003) EMBO J 22, 6089-100. 4 . Banting, G.S. et al. (2005) Hum Mol Genet 14, 513-24. 5 . Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84. 6 . Landry, J.W. et al. (2011) Genes Dev 25, 275-86. 7 . Landry, J. et al. (2008) PLoS Genet 4, e1000241. |
Western blot analysis of extracts from various cell lines using SMARCA1 Antibody. 使用SMARCA1 Antibody，免疫印迹(Western blot)分析不同细胞中SMARCA1的蛋白水平。 |
