| 货号 | 9513S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Smad3 Antibody detects endogenous levels of total Smad3 protein. |
| 使用方法 | WB(1:1000) IP (1:25) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
| 参考文献 | 1 . Heldin, C.H. et al. (1997) Nature 390, 465-71. 2 . Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94. 3 . Derynck, R. et al. (1998) Cell 95, 737-40. 4 . Massagué, J. (1998) Annu Rev Biochem 67, 753-91. 5 . Whitman, M. (1998) Genes Dev 12, 2445-62. 6 . Wu, G. et al. (2000) Science 287, 92-7. 7 . Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7. 8 . Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69. 9 . Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685. 10 . Souchelnytskyi, S. et al. (1997) J. Biol. Chem. 272, 28107-28115. 11 . Liu, X. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10669-10674. |
Western blot analysis of extracts from HT1080, HeLa and B35 cell lines, using Smad3 Antibody. 使用Smad3抗体对Ht1080，HeLa细胞和B35细胞系细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa cells transfected with Smad3, using Smad3 Antibody. 使用Smad3抗体对转染了Smad3的HeLa细胞提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of HT1080 cells, serum-starved (left), or treated with hTGF-β3 #8425 (100 ng/ml, 30 min; right), using Smad3 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). 血清饥饿(左)或经hTGF-β3 #8425 (100 ng/ml, 30 min; 右)处理HT1080细胞，使用Smad3 抗体 (绿色)进行共聚焦免疫荧光进行分析。肌动蛋白丝使用DY-554 phalloidin (红色)进行标记。 |
