| 货号 | 3102S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Smad2 Antibody detects endogenous levels of total Smad2/3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 52, 60 |
| 参考文献 | 1 . Heldin, C.H. et al. (1997) Nature 390, 465-71. 2 . Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94. 3 . Derynck, R. et al. (1998) Cell 95, 737-40. 4 . Massagué, J. (1998) Annu Rev Biochem 67, 753-91. 5 . Whitman, M. (1998) Genes Dev 12, 2445-62. 6 . Wu, G. et al. (2000) Science 287, 92-7. 7 . Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7. 8 . Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69. 9 . Abdollah, S. et al. (1997) J. Biol. Chem. 272, 27678-27685. 10 . Souchelnytskyi, S. et al. (1997) J. Biol. Chem. 272, 28107-28115. 11 . Liu, X. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 10669-10674. |
Western blot analysis of various cell lines using Smad2/3 Antibody. 使用Smad2/3 抗体对多种细胞提取物进行western blot分析。 |
