| 货号 | 9743S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/ChIP |
| 目标/特异性 | Smad1 Antibody detects endogenous levels of total Smad1 protein. No cross reactivity was observed with other family members. |
| 使用方法 | WB(1:1000) IP (1:100) ChIP (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | -20C |
| 计算分子量 | 58-60 |
| 参考文献 | 1 . Hogan, B.L. (1996) Genes Dev 10, 1580-94. 2 . Hoodless, P.A. et al. (1996) Cell 85, 489-500. 3 . Klemm, J.D. et al. (1998) Annu Rev Immunol 16, 569-92. 4 . Kretzschmar, M. et al. (1997) Genes Dev 11, 984-95. 5 . Whitman, M. (1998) Genes Dev 12, 2445-62. 6 . Sapkota, G. et al. (2007) Mol Cell 25, 441-54. 7 . Alarcón, C. et al. (2009) Cell 139, 757-69. |
Western blot analysis of extracts from various cell lines using Smad1 Antibody. 使用Smad1抗体对多种细胞提取物进行western blot实验。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells treated with Human BMP2 #4697 (50 ng/ml) for one hour and either 20 μl of Smad1 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ID1 Promoter Primers #5139, human SMAD6 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将经过人BMP2 #4697 (50 ng/ml)处理1小时的4 x 106 MCF7细胞和20μl Smad1抗体或2μl Normal Rabbit IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人ID1启动子引物#5139，人SMAD6启动子引物和SimpleChIP®人α Satellite Repeat引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量对比于input染色质总量进行相对定量，染色质量设定值为1. |
