| 货号 | 12532S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Siva-1 Antibody recognizes endogenous levels of total Siva-1 protein. This antibody does not cross-react with Siva-2. This antibody cross-reacts with a protein of unknown origin at ~70 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | First identified as a pro-apoptotic protein that binds the cytoplasmic tail of the TNF receptor superfamily member CD27 (1), Siva-1 also binds several other TNFR family members including glucocorticoid-induced tumor necrosis factor receptor (GITR) and OX40 (1-3), as well as anti-apoptotic Bcl-2 family members Bcl-xL and Bcl-2 (4,5). Siva-1 is composed of a central death domain homology region, a C-terminal box-B-like ring finger followed by a zinc finger-like domain, and a unique N-terminal amphipathic helical region (SAH) (1,4). Studies have demonstrated that Siva-1 has the ability to induce cell death via both the extrinsic and intrinsic apoptotic pathways (1-8). The SAH domain of Siva-1 is responsible for the inhibition of the pro-survival activities of Bcl-xL and Bcl-2, leading to caspase-mediated cell death (4,5,8). Siva-1 plays a role in T cell signaling and homeostasis by inhibiting NF-κB activity, also resulting in apoptotic cell death (7,9). An alternative splice variant of Siva-1, Siva-2, lacks part of the SAH and death domains and is less effective at inducing apoptosis (1,2,5,8). Studies in xenografts have shown that down-regulation of Siva-1 inhibits tumorigenesis in response to p53 activation (10). Down-regulation of Siva-1 may also play a role in tumor metastasis through its regulation of the epithelial-mesenchymal transition (EMT) and cell migration (11). Overexpression of Siva-1 is implicated in several pathological conditions including acute ischemic injury (12) and Coxsackievirus infection (13). |
| 存放说明 | -20C |
| 计算分子量 | 19 |
| 参考文献 | 1 . Prasad, K.V. et al. (1997) Proc Natl Acad Sci U S A 94, 6346-51. 2 . Yoon, Y. et al. (1999) Oncogene 18, 7174-9. 3 . Spinicelli, S. et al. (2002) Cell Death Differ 9, 1382-4. 4 . Xue, L. et al. (2002) Proc Natl Acad Sci U S A 99, 6925-30. 5 . Chu, F. et al. (2004) Apoptosis 9, 83-95. 6 . Cao, C. et al. (2001) J Biol Chem 276, 11465-8. 7 . Gudi, R. et al. (2006) Oncogene 25, 3458-62. 8 . Py, B. et al. (2004) J Immunol 172, 4008-17. 9 . Hench, V.K. and Su, L. (2011) BMC Immunol 12, 54. 10 . Du, W. et al. (2009) Cell Death Differ 16, 1493-504. 11 . Li, N. et al. (2011) Proc Natl Acad Sci U S A 108, 12851-6. 12 . Padanilam, B.J. et al. (1998) Kidney Int 54, 1967-75. 13 . Henke, A. et al. (2000) J Virol 74, 4284-90. |
Immunoprecipitation of Siva-1 from MOLT-4 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Siva-1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Siva-1 Antibody. 免疫沉淀方法检测MOLT-4细胞提取物中Siva-1，使用抗体为Normal Rabbit IgG #2729 (泳道2)或Siva-1 Antibody (泳道3)。泳道1为10%对照。使用Siva-1 Antibody 进行Western blot分析。 | |
Western blot analysis of extracts from MOLT-4, HBP-ALL, and CCRF-CEM cells using Siva-1 Antibody. Western blot检测MOLT-4、HBP-ALL和CCRF-CEM 细胞系提取物，使用抗体为 Siva-1 Antibody。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-), transfected with a construct expressing Myc/DDK-tagged full-length human Siva-1 (hSiva-1-Myc/DDK; +), or transfected with a construct expressing Myc/DDK-tagged full-length human Siva-2 (hSiva-2-Myc/DDK; +), using Siva-1 Antibody or Myc-Tag (71D10) Rabbit mAb #2278. Western blot方法检测293T细胞提取物：空质粒转染（-）或带Myc/DDK-标签全长人Siva-1 (hSiva-1-Myc/DDK; +)或Myc/DDK-标签全长人Siva-2 (hSiva-2-Myc/DDK; +)载体转染；使用抗体为Siva-1 Antibody或Myc-Tag (71D10) Rabbit mAb 兔单抗#2278。 |
