| 货号 | 2590S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SirT6 Antibody detects endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2 (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Guarente, L. (1999) Nat. Genet. 23, 281-285. 2 . Mostoslavsky, R. et al. (2006) Cell 124, 315-29. 3 . Liszt, G. et al. (2005) J Biol Chem 280, 21313-20. 4 . Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35. |
Western blot analysis of various cell types using SirT6 Antibody. 使用SirT6 Antibody，免疫印迹(Western blot)分析不同细胞中SirT6的蛋白水平。 |
