| 货号 | 2028S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | SirT1 Antibody (Mouse Specific) detects endogenous levels of total SirT1 protein. This antibody does not cross-react with other sirtuin proteins. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7) and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
| 参考文献 | 1 . Guarente, L. (1999) Nat. Genet. 23, 281-285. 2 . Vaziri H et al. (2001) Cell 107, 149–59 3 . Luo, J. et al. (2001) Cell 107, 137-148. 4 . Bouras T et al. (2005) J Biol Chem 280, 10264–76 5 . Brunet, A. et al. (2004) Science 303, 2011-5. 6 . Motta, M.C. et al. (2004) Cell 116, 551-563. 7 . Picard, F. et al. (2004) Nature 429, 771-776. 8 . Rodgers, J.T. et al. (2005) Nature 434, 113-8. 9 . Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci U S A 101, 12130-5. |
Western blot analysis of extracts from F9, C2C12 and PYS-2 cells using SirT1 Antibody (Mouse Specific). 使用SirT1 Antibody (Mouse Specific)，免疫印迹(Western blot)分析F9、C2C12和PYS-2细胞中SirT1蛋白水平。 | |
Confocal immunofluorescent analysis of C2C12 cells using SirT1 Antibody (Mouse Specific) (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用SirT1 Antibody (Mouse Specific) (绿色)标记，共聚焦免疫荧光分析C2C12细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
