| 货号 | 3279S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Shootin1 Antibody detects endogenous levels of total shootin1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The differentiation process of neurons can be divided into five stages, each stage characterized by morphological changes observed in the developing cells. In stage 1, the cells extend lamellipodia and in stage 2 their lamellipodia develop into immature neurites. In stage 3 one neurite elongates rapidly to form an axon and in stage 4 the remaining immature neuritis elongate to form dendrites. In stage 5 synaptic contacts are formed and a neuronal network is established (1). Shootin1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3. The extension of an axon requires considerable reorganization of the cytoskeleton mediated by PI3K/Akt and PI3K/Cdc42 signaling (1). Shootin1 is involved in regulating the subcellular localization of PI3 kinase. Furthermore, shootin1 is upregulated during polarization and accumulates asymmetrically in a single neurite that consequently elongates rapidly to form an axon (2). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Yoshimura, T. et al. (2006) J Neurosci 26, 10626-30. 2 . Toriyama, M. et al. (2006) J Cell Biol 175, 147-57. |
Western blot analysis of extracts from neonatal mouse and fetal rat brain using Shootin1 Antibody. Western blot分析新生小鼠和胎大鼠脑提取物，使用的抗体是Shootin1 Antibody 兔多抗。 |
