| 货号 | 12762S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SHMT2 Antibody recognizes endogenous levels of total SHMT2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxylmethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5, 10-methylene-THF (1, 2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
| 参考文献 | 1 . MacFarlane, A.J. et al. (2008) J Biol Chem 283, 25846-53. 2 . Hebbring, S.J. et al. (2012) J Neurochem 120, 881-90. 3 . Macfarlane, A.J. et al. (2011) Cancer Res 71, 2098-107. 4 . di Salvo, M.L. et al. (2013) Med Hypotheses 80, 633-6. |
Western blot analysis of extracts from various cell lines using SHMT2 Antibody. 使用SHMT2 Antibody对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing either Myc/DDK-tagged full-length human SHMT1 (hSHMT1-Myc/DDK; +) or Myc/DDK-tagged full-length human SHMT2 (hSHMT2-Myc/DDK; +), using SHMT2 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). 模拟转染(-)或转染了表达Myc/DDK标记full-length human SHMT1 (hSHMT1-Myc/DDK; +) 或 Myc/DDK-tagged full-length human SHMT2 (hSHMT2-Myc/DDK; +)质粒的293细胞提取物，使用SHMT2 Antibody (上), DYKDDDDK Tag (9A3) Mouse mAb鼠单抗 #8146 (中)或 GAPDH (D16H11) XP® Rabbit mAb兔单抗 #5174 (下)进行western blot分析。 |
