| 货号 | 2730S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SHIP2 Antibody detects the endogenous levels of total SHIP2 protein. It does not cross-react with SHIP1. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
| 参考文献 | 1 . Tridandapani, S. et al. (1997) Mol. Cell. Biol. 17, 4305-4311. 2 . Liu, L. et al. (1997) J Biol Chem 272, 8983-8. 3 . Malbec, O. et al. (2001) J Biol Chem 276, 30381-91. 4 . Carver, D.J. et al. (2000) Blood 96, 1449-56. 5 . Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72. 6 . Sattler, M. et al. (2001) J Biol Chem 276, 2451-8. 7 . Pesesse, X. et al. (1997) Biochem Biophys Res Commun 239, 697-700. 8 . Wada, T. et al. (2001) Mol Cell Biol 21, 1633-46. 9 . Ishida, S. et al. (2006) Pancreas 33, 63-7. 10 . Dyson, J.M. et al. (2005) Int J Biochem Cell Biol 37, 2260-5. 11 . Sasaoka, T. et al. (2006) Pharmacol Ther 112, 799-809. |
Western blot analysis of total cell lysates from SKMEL5 and HeLa cells, using SHIP2 Antibody.Western免疫印迹。用SHIP2 Antibody研究SKMEL5和HeLa 细胞的细胞提取液。 |
