| 货号 | 2728S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SHIP1 (D1163) Antibody detects the endogenous levels of total SHIP1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
| 参考文献 | 1 . Tridandapani, S. et al. (1997) Mol. Cell. Biol. 17, 4305-4311. 2 . Liu, L. et al. (1997) J Biol Chem 272, 8983-8. 3 . Malbec, O. et al. (2001) J Biol Chem 276, 30381-91. 4 . Carver, D.J. et al. (2000) Blood 96, 1449-56. 5 . Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72. 6 . Sattler, M. et al. (2001) J Biol Chem 276, 2451-8. |
Western blot analysis of total cell lysates from RL, BaF3 and BW5147 cells, using SHIP1 (D1163) Antibody.Western免疫印迹。用SHIP1 (D1163) Antibody研究RL，BaF3 和BW5147 细胞的细胞提取液。 |
