| 货号 | 2271S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Shh/Ihh antibody detects both the precursor and mature forms of transfected Shh and transfected Ihh. It is also expected to recognize Shh from cat, dog, chicken and zebra fish based on homology. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 19, (42, 45) |
| 参考文献 | 1 . Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87. 2 . McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114. 3 . Zhang, X.M. et al. (2001) Cell 106, 781-92. 4 . Adolphe, C. et al. (2004) Development 131, 5009-19. 5 . Pathi, S. et al. (2001) Mech Dev 106, 107-17. 6 . Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394. |
Western blot analysis of total cell lysates from 293 cells and 293 cells transiently transfected with mouse Shh or Ihh construct, using Shh/Ihh Antibody. 使用Shh/Ihh 抗体对293细胞和瞬转了小鼠Shh或Ihh载体的293细胞提取物进行western blot分析。 |
