| 货号 | 2432S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Shc Antibody detects endogenous levels of total Shc protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7). |
| 存放说明 | -20C |
| 计算分子量 | 46, 52, 66 |
| 参考文献 | 1 . Pelicci, G. et al. (1992) Cell 70, 93-104. 2 . Migliaccio, E. et al. (1997) EMBO J. 16, 706-716. 3 . Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049. 4 . Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836. 5 . Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204. 6 . Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444. 7 . Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755. |
Western blot analysis of extracts from HepG2 and Jurkat cells, using Shc Antibody.Western blot方法检测细胞提取物：Jurkat 、 HepG2细胞。使用的抗体是Shc antibody. |
