| 货号 | 12218S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (6). |
| 存放说明 | -20C |
| 计算分子量 | 165 |
| 参考文献 | 1 . Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603. 2 . Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18. 3 . Boeckers, T.M. et al. (2005) J Neurochem 92, 519-24. 4 . Sala, C. et al. (2001) Neuron 31, 115-30. 5 . Boeckers, T.M. et al. (2002) J Neurochem 81, 903-10. 6 . Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603. |
Western blot analysis of extracts from mouse and rat brain tissue using SHANK2 Antibody. Western blot 检测小鼠和大鼠大脑组织提取物，使用的抗体是SHANK2 Antibody兔多抗。 | |
Western blot analysis of extracts from the indicated rat brain synaptic fractions using SHANK2 Antibody. Separation of the different synaptic fractions was confirmed using PSD95 (D27E11) XP® Rabbit mAb #3450 and Syntaxin 6 (C34B2) Rabbit mAb #2869. Equal loading of each fraction was assessed using β-Tubulin (9F3) Rabbit mAb #2128. Fractionation of the different synaptic compartments was carried out as described by Phillips, G.R. et al. (2001) Neuron 32, 63-77. PAZ, Pre-synaptic active zone. Western blot 检测指定大鼠脑突触组分提取物，使用的抗体是SHANK2 Antibody兔多抗。使用抗体PSD95 (D27E11) XP® Rabbit mAb兔单抗 #3450和Syntaxin 6 (C34B2) Rabbit mAb兔单抗 #2869检测不同突触组分的分离。使用抗体 β-Tubulin (9F3) Rabbit mAb兔单抗 #2128评估各个组分内参的调平。不同突触组分的处理按照以下文献描述进行。Phillips, G.R. et al. (2001) Neuron 32, 63-77. PAZ, Pre-synaptic active zone。 |
