| 货号 | 4690S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | SFRP1 Antibody detects endogenous levels of total SFRP1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Secreted Frizzled-related proteins (SFRPs) display homology and structural similarity to the extracellular cysteine-rich Wnt-binding domain of the G protein-coupled receptor Frizzled (1,2). To date, five distinct SFRPs (SFRP1 to 5) have been found in mammalian cells. These secreted proteins typically act as antagonists to Wnt signaling by directly binding and inhibiting Wnt proteins, or by binding Frizzled to block Wnt protein interaction with the receptor (3). The various SFRPs bind and regulate Wnt proteins differentially; these proteins also display distinct expression patterns as they play important roles in regulating development (4-7). SFRP proteins appear to act as tumor suppressors, with loss of expression or function correlating with many invasive forms of cancer. Deletion of the corresponding SFRP1 gene and promoter hypermethylation leading to gene silencing has been reported in a number of cancers. Abnormal expression of SRFP1 and other Wnt signaling proteins is associated with some cases of retinitis pigmentosa (reviewed in 8). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
| 参考文献 | 1 . Dann, C.E. et al. (2001) Nature 412, 86-90. 2 . Kawano, Y. and Kypta, R. (2003) J Cell Sci 116, 2627-34. 3 . Bafico, A. et al. (1999) J Biol Chem 274, 16180-7. 4 . Galli, L.M. et al. (2006) Dev Dyn 235, 681-90. 5 . Wawrzak, D. et al. (2007) Biochem Biophys Res Commun 357, 1119-23. 6 . Hu, E. et al. (1998) Biochem Biophys Res Commun 247, 287-93. 7 . Mao, C. et al. (2000) Arterioscler Thromb Vasc Biol 20, 43-51. 8 . Bovolenta, P. et al. (2008) J Cell Sci 121, 737-46. |
Western blot analysis of extracts from U118MG and SK-MEL-28 cells using SFRP1 Antibody.使用SFRP1抗体对U118MG和SK-MEl-28细胞提取物进行western blot分析。 |
