| 货号 | 9678S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Sec24A Antibody detects endogenous levels of total Sec24A protein. This antibody does not cross-react with other members of Sec24 family. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Coat Protein Complex II (COPII) is composed of five cytosolic proteins: Sec23/24 complex, Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex, thereby forming a prebudding complex that directly binds target molecules (1-3). The prebudding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec24 subunit of COPII coat is thought to play a critical role in cargo selection (2,6). It binds directly to cargo proteins at the ER and brings them to COPII vesicles through interaction with Sec23. There are four Sec24 isoforms in human cells: Sec24A, Sec24B, Sec24C, and Sec24D (7). In mice, mutations in Sec24B have been linked to developmental defects (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
| 参考文献 | 1 . Aridor, M. et al. (1998) J Cell Biol 141, 61-70. 2 . Miller, E.A. et al. (2003) Cell 114, 497-509. 3 . Mossessova, E. et al. (2003) Cell 114, 483-95. 4 . Barlowe, C. et al. (1994) Cell 77, 895-907. 5 . Bi, X. et al. (2007) Dev Cell 13, 635-45. 6 . Miller, E. et al. (2002) EMBO J 21, 6105-13. 7 . Tang, B.L. et al. (1999) Biochem Biophys Res Commun 258, 679-84. 8 . Merte, J. et al. (2010) Nat Cell Biol 12, 41-6; sup pp 1-8. 9 . Wansleeben, C. et al. (2010) Development 137, 1067-73. |
Western blot analysis of extracts from various cell lines using Sec24A Antibody.使用Sec24A抗体对多种细胞提取物进行western blot分析。 |
