| 货号 | 4475S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Scribble Antibody detects endogenous levels of total scribble protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Scribble (Scrib) was originally identified in a genetic screen in Drosophila along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). Drosophila mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds β-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8). |
| 存放说明 | -20C |
| 计算分子量 | 240 |
| 参考文献 | 1 . Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80. 2 . Bilder, D. et al. (2000) Science 289, 113-6. 3 . Humbert, P.O. et al. (2008) Oncogene 27, 6888-907. 4 . Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400. 5 . Qin, Y. et al. (2005) J Cell Biol 171, 1061-71. 6 . Navarro, C. et al. (2005) Oncogene 24, 4330-9. 7 . Takizawa, S. et al. (2006) Genes Cells 11, 453-64. 8 . Nguyen, M.L. et al. (2003) J Virol 77, 6957-64. |
Western blot analysis of extracts from various cell lines using Scribble Antibody.使用Scribble抗体对多种细胞提取物进行western blot分析。 |
