| 货号 | 2438S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | SCD1 (M38) Antibody detects endogenous levels of total SCD1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl–CoA and stearoyl–CoA to palmitoleoyl–CoA (16:1) and oleoyl–CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
| 参考文献 | 1 . Ntambi, J.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11482-114866. 2 . Kato, H. et al. (2006) J. Cell. Sci. 119, 2342-2353. 3 . Ozols, J. (1997) Mol. Biol. Cell 8, 2281-2290. 4 . Rahman SM et al. (2005) Am J Physiol Endocrinol Metab 288, E381–7 5 . Gutiérrez-Juárez, R. et al. (2006) J. Clin. Invest. 116, 1686-1695. |
Confocal immunofluorescent analysis of 3T3-L1 cells, using SCD1 (M38) Antibody (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对3T3-L1细胞使用SCD1 (M38) Antibody（红色）进行共聚焦免疫荧光分析，显示分化细胞的胞质定位。脂质小滴被BODIPY 493/503 (绿色)标记。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Western blot analysis of 3T3-L1 cell lysates, using SCD1 (M38) Antibody. 对3T3-L1细胞裂解液使用SCD1 (M38) Antibody进行Western blot分析。 |
