| 货号 | 13102S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SCAP Antibody recognizes endogenous levels of total SCAP protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SREBP cleavage activating protein (SCAP) is a membrane-bound protein essential in regulating sterol regulatory element binding protein (SREBP) processing (1). In cholesterol-starved cells, SREBPs move from the ER to the Golgi apparatus with the help of SCAP, where the precursor SREBPs are cleaved by site-1 (S1P) and site-2 (S2P) proteases (1,2). Released SREBP amino-terminal domains enter the nucleus and bind to sterol response elements located in the promoters of a number of genes responsible for the cholesterol synthesis (1,2). Research studies show that the SCAP/SREBP pathway is critical for diabetic fatty liver development and carbohydrate-induced hypertriglyceridemia in mice (3). In addition, the SCAP/SREBP pathway is required to protect cancer cells from lipotoxicity (4). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Brown, M.S. and Goldstein, J.L. (1997) Cell 89, 331-40. 2 . Horton, J.D. et al. (2002) J Clin Invest 109, 1125-31. 3 . Moon, Y.A. et al. (2012) Cell Metab 15, 240-6. 4 . Williams, K.J. et al. (2013) Cancer Res 73, 2850-62. |
Western blot analysis of extracts from various cell lines using SCAP Antibody. 使用SCAP Antibody对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SCAP (hSCAP-Myc/DDK; +), using SCAP Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). 模拟转染(-)或转染了表达Myc/DDK标记的full-length human SCAP 质粒(hSCAP-Myc/DDK; +)的293细胞提取物，使用SCAP Antibody (上), Myc-Tag (71D10) Rabbit mAb兔单抗 #2278 (中), 和β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下)进行western blot分析。 |
