| 货号 | 3507S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Sav1 Antibody detects endogenous level of total Sav1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sav1 (Salvador homolog 1) was first identified as a 45 kDa protein containing a pair of WW domains and a coiled-coil region. Originally named WW45, Sav1 is ubiquitously expressed in adult tissues but is also present during specific developmental periods. Sav1 acts a scaffold protein in a complex that includes the kinases MST2 and LATS1, and a YAP transcriptional co-activator (3). This group of proteins functions in the newly identified Hippo pathway, which regulates important developmental functions such as cell contact inhibition and organ size. Further evidence suggests a role in tumor suppression and cancer pathogenesis (4,5). The Hippo pathway requires Sav1 for activation of MST1 and the resulting activation of LATS1/2 (6). Embryonic mice lacking Sav1 display hyperplastic growth and epithelial differentiation defects, implying that Sav1 plays a prominent role epithelial cell differentiation (7). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Valverde, P. (2000) Biochem Biophys Res Commun 276, 990-8. 2 . Oka T et al. (2008) J Biol Chem 283, 27534–46 3 . Guo, C. et al. (2007) Curr Biol 17, 700-5. 4 . Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92. 5 . Tapon, N. et al. (2002) Cell 110, 467-78. 6 . Lee, J.H. et al. (2008) EMBO J 27, 1231-42. 7 . Donninger, H. et al. (2011) J Biol Chem 286, 18483-91. |
Western blot analysis of extracts from various cell types using Sav1 Antibody.使用Sav1抗体对多种细胞提取物进行western blot分析。 |
