| 货号 | 3650S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | SATB1 (L745) Antibody detects endogenous levels of total SATB1 protein. |
| 使用方法 | WB(1:1000) IP (1:25) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Special AT-rich binding protein 1 (SATB1) functions as both a global chromatin organizer and a gene-specific transcription factor (1). SATB1 cooperates with promyelocytic leukemia protein (PML) to regulate global chromatin architecture by organizing chromatin into distinct loops via periodic anchoring of matrix attachment regions (MARs) in DNA to the nuclear matrix (1-3). In addition, SATB1 recruits multiple chromatin-remodeling proteins that contribute to specific gene activation and repression, including the chromatin remodeling enzymes ACF and ISWI, the histone deacetylase HDAC1, and the histone acetyltransferases PCAF and p300/CBP (4-6). Phosphorylation of SATB1 on Ser185 by protein kinase C regulates its interaction with HDAC1 and PCAF. While unphosphorylated SATB1 binds to PCAF, phosphorylated SATB1 preferentially binds to HDAC1 (6). Acetylation of SATB1 on Lys136 by PCAF impairs its DNA binding activity, thereby removing SATB1 from gene promoters (6). SATB1 is expressed predominantly in thymocytes and is involved in gene regulation during T cell activation (1). SATB1 is also expressed in metastatic breast cancer cells and is a potential prognostic marker and therapeutic target for metastatic breast cancer (7). In a mouse model system, RNAi-mediated knockdown of SATB1 reversed tumorigenesis by inhibiting tumor growth and metastasis, while ectopic expression of SATB1 in non-metastatic breast cancer cells produced invasive tumors. |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Galande, S. et al. (2007) Curr Opin Genet Dev 17, 408-14. 2 . Cai, S. et al. (2006) Nat Genet 38, 1278-88. 3 . Kumar, P.P. et al. (2007) Nat Cell Biol 9, 45-56. 4 . Yasui, D. et al. (2002) Nature 419, 641-5. 5 . Kumar, P.P. et al. (2005) Mol Cell Biol 25, 1620-33. 6 . Pavan Kumar, P. et al. (2006) Mol Cell 22, 231-43. 7 . Han, H.J. et al. (2008) Nature 452, 187-93. |
Western blot analysis of extracts from Jurkat and THP-1 cell lines using SATB1 (L745) Antibody. 使用SATB1 (L745) Antibody，免疫印迹(Western blot)分析Jurkat和THP-1细胞中SATB1 (L745)的蛋白水平。 | |
Confocal immunofluorescent analysis of HEK/293 cells using SATB1 (L745) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用SATB1 (L745) Antibody (绿色)，共聚焦免疫荧光分析HEK/293细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
