| 货号 | 12361S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SAMHD1 Antibody recognizes endogenous levels of total SAMHD1 protein. This antibody cross-reacts with a 30 kDa protein of unknown origin. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SAM domain and HD domain-containing protein 1 (SAMHD1) is a negative regulator of the cell-intrinsic innate immune response (1). Research studies have identified mutations in SAMHD1 as a cause of Aicardi-Goutieres syndrome, an autoimmune disease characterized by elevated production of interferon-α and symptoms resembling congenital viral infection (1). Recently, SAMHD1 was identified as the restriction factor that renders most myeloid cells refractory to infection by human immunodeficiency virus (HIV) (2-4). Expression of the viral protein Vpx in refractory cells targets SAMHD1 for ubiquitin-mediated degradation and relieves HIV restriction. SAMHD1 prevents autoimmunity and HIV infection by hydrolizing intracellular deoxynucleoside triphosphates (dNTPs), thereby limiting inappropriate immune activation by self nucleic acid and inhibiting reverse transcription of the HIV genome (4-6).SAM结构域和HD结构域蛋白1（SAMHD1）是细胞内在的先天性免疫应答的“负调节器”（1）。研究表明SAMHD1突变能引起Aicardi-Goutieres综合征，这是一种自身免疫病，以干扰素α增加为特征，症状类似于先天性病毒感染（1）。最近，SAMHD1被认为是一种限制因子，使许多髓系细胞能够抵抗人免疫缺陷病毒（HIV）的感染（2-4）。抗感染细胞中病毒蛋白Vpx的表达能使SAMHD1进行由泛素介导的降解，解除对HIV的限制。SAMHD1通过水解细胞内的三磷酸脱氧核糖核苷(dNTPs)来防止自身免疫和HIV感染，限制自身核酸引起的不适当的免疫激活以及抑制HIV基因组的反转录（4-6）。 |
| 存放说明 | -20C |
| 计算分子量 | 69, 72 |
| 参考文献 | 1 . Rice, G.I. et al. (2009) Nat Genet 41, 829-32. 2 . Laguette, N. et al. (2011) Nature 474, 654-7. 3 . Hrecka, K. et al. (2011) Nature 474, 658-61. 4 . Powell, R.D. et al. (2011) J Biol Chem 286, 43596-600. 5 . Goldstone, D.C. et al. (2011) Nature 480, 379-82. 6 . Lahouassa, H. et al. (2012) Nat Immunol 13, 223-8. |
Western blot analysis of extracts from various cell lines using SAMHD1 Antibody.Western blot分析来自于不同细胞系的提取物，使用的抗体是SAMHD1 Antibody。 |
