| 货号 | 5066S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | S100A1 Antibody recognizes endogenous levels of total S100A1 protein. This antibody does not cross-react with other members of the S100 family. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Despite their relatively small size (8-12 kDa) and uncomplicated architecture, S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. To date, 25 members have been identified, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z, making it the largest group in the EF-hand, calcium-binding protein family. Interestingly, 14 S100 genes are clustered on human chromosome 1q21, a region of genomic instability. Research studies have demonstrated significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development (1-4). Each S100 monomer bears two EF-hand motifs and can bind up to two molecules of calcium (or other divalent cation in some instances). Structural evidence shows that S100 proteins form antiparallel homo- or heterodimers that coordinate binding partner proximity in a calcium-dependent (and sometimes calcium-independent) manner. Although structurally and functionally similar, individual members show restricted tissue distribution, are localized in specific cellular compartments, and display unique protein binding partners that suggest each plays a specific role in various signaling pathways. In addition to an intracellular role, some S100 proteins have been shown to act as receptors for extracellular ligands or are secreted and exhibit cytokine-like activities (1-4). |
| 存放说明 | -20C |
| 计算分子量 | 12 |
| 参考文献 | 1 . Heizmann, C.W. et al. (2002) Front Biosci 7, d1356-68. 2 . Donato, R. (2003) Microsc Res Tech 60, 540-51. 3 . Marenholz, I. et al. (2004) Biochem Biophys Res Commun 322, 1111-22. 4 . Santamaria-Kisiel, L. et al. (2006) Biochem J 396, 201-14. 5 . Ritterhoff, J. and Most, P. (2012) Gene Ther , . 6 . Völkers, M. et al. (2010) J Biomed Biotechnol 2010, 178614. 7 . Prosser, B.L. et al. (2011) Cell Calcium 50, 323-31. 8 . Wright, N.T. et al. (2009) Curr Chem Biol 3, 138-145. |
Western blot analysis of extracts from various cell lines using S100A1 Antibody.Western blot方法检测多个细胞系提取物，使用的抗体为S100A1 Antibody. | |
Western blot analysis of recombinant, purified S100 family proteins (50 ng) using S100A1 Antibody (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).Western blot方法检测重组纯化的S100家族蛋白(50 ng)，使用的抗体为S100A1 Antibody (上图)或Myc-Tag (71D10) Rabbit mAb #2278 (下图)。 |
