| 货号 | 5388S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | RXRα Antibody recognizes endogenous levels of total RXRα protein. RXRα Antibody does not cross-react with either RXRβ or RXRγ. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).人视黄醇X受体（RXRs）由三种不同的基因（RXRα，RXRβ，RXRγ）编码，并与具有高亲和力的维生素A衍生物，9-顺-视黄酸选择性结合。 RXRs是II型核激素受体，主要位于核室，与非依赖性配体结合。核RXR与核激素受体亚族蛋白形成异二聚体，包括甲状腺激素受体、维甲酸受体、维生素D受体、过氧化物酶体增殖物活化受体、肝脏X受体、法尼酯X受体（1）。由于RXR与多个核激素受体形成异源二聚体，通过与靶基因启动子/增强区域的顺式作用元件结合，在众多激素信号转导通路的转录调控中发挥核心作用（2）。 |
| 存放说明 | -20C |
| 计算分子量 | 53 |
| 参考文献 | 1 . Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64. 2 . Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44. 3 . Mangelsdorf, D.J. et al. (1990) Nature 345, 224-9. 4 . Dollé, P. et al. (1994) Mech Dev 45, 91-104. 5 . Kastner, P. et al. (1994) Cell 78, 987-1003. 6 . Sucov, H.M. et al. (1994) Genes Dev 8, 1007-18. 7 . Macoritto, M. et al. (2008) J Biol Chem 283, 4943-56. |
Western blot analysis of extracts from various cell lines using RXRα Antibody.Western blot方法检测多个细胞系提取物，使用的抗体为RXRα Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected or transfected with human RXRα, RXRβ, RXRγ DYKDDDDK tagged constructs, using RXRα Antibody (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (lower).Western blot方法检测293T细胞提取物，细胞不转染或用DYKDDDDK标记的人RXRα, RXRβ, RXRγ构建体转染，使用的抗体为RXRα Antibody (上图) 和DYKDDDDK Tag Antibody (与Sigma的 Anti-FLAG® M2 Antibody结合相同的抗原表位) #2368 (下图). |
