| 货号 | 4742S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | RKIP Antibody detects endogenous levels of total RKIP protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-κB pathway, including NF-κB-inducing kinase (NIK), TAK1, IKKα and IKKβ (5). These interactions antagonize cytokine-induced activation of the NF-κB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
| 参考文献 | 1 . Yeung, K. et al. (1999) Nature 401, 173-7. 2 . Yeung, K. et al. (2000) Mol Cell Biol 20, 3079-85. 3 . Corbit, K.C. et al. (2003) J Biol Chem 278, 13061-8. 4 . Lorenz, K. et al. (2003) Nature 426, 574-9. 5 . Yeung, K.C. et al. (2001) Mol Cell Biol 21, 7207-17. 6 . Fu, Z. et al. (2003) J Natl Cancer Inst 95, 878-89. |
Western blot analysis of extracts from COS, C6, NIH/3T3, HeLa and A431 cells, using RKIP Antibody. |
