RIP4 Antibody【科瑞奥博】

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RIP4 Antibody

货号： 12636S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	12636S
反应种属	Human
来源宿主	Rabbit
应用	W/IP
目标/特异性	RIP4 Antibody recognizes endogenous levels of total RIP4 protein.
使用方法	WB(1:1000) IP (1:50)

性能
供应商	CST
灵敏度	Endogenous
背景	The receptor-interacting protein (RIP) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that trigger pro-survival and inflammatory responses through the activation of NF-κB, as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and recruitment to TNF-R1 through interaction with TRADD (2,3). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (4,5). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8-dependent cleavage of the RIP death domain can trigger the apoptotic activity of RIP (8).
存放说明	-20C
计算分子量	86
参考文献	1 . Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9. 2 . Hsu, H. et al. (1996) Immunity 4, 387-96. 3 . Stanger, B.Z. et al. (1995) Cell 81, 513-23. 4 . Ting, A.T. et al. (1996) EMBO J 15, 6189-96. 5 . Kelliher, M.A. et al. (1998) Immunity 8, 297-303. 6 . Devin, A. et al. (2000) Immunity 12, 419-29. 7 . Zhang, S.Q. et al. (2000) Immunity 12, 301-11. 8 . Lin, Y. et al. (1999) Genes Dev 13, 2514-26. 9 . Bhr, C. et al. (2000) J Biol Chem 275, 36350-7. 10 . Chen, L. et al. (2001) J Biol Chem 276, 21737-44. 11 . Rountree, R.B. et al. (2010) J Invest Dermatol 130, 102-12. 12 . Adams, S. et al. (2007) J Invest Dermatol 127, 538-44. 13 . Holland, P. et al. (2002) Curr Biol 12, 1424-8. 14 . Huang, X. et al. (2013) Science 339, 1441-5.

参考图片

Western blot analysis of extracts from PANC-1, Malme-3M, and 786-O cells using RIP4 Antibody.Western blot分析PANC-1,Malme-3M和786-O细胞的细胞提取物，使用抗体是RIP4抗体。

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RIP4 isoform 2 (hRIP4-Myc/DDK; +), using RIP4 Antibody.Western blot分析293T细胞的细胞提取物，对照转染(-)或转染表达Myc/DDK标签的全长人源isoform2 构建质粒(hRIP4-Myc/DDK; +),使用抗体是RIP4抗体。

Immunoprecipitation of RIP4 from PANC-1 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or RIP4 Antibody (lane 3). Lane 1 is 10% input. Western blot was performed using RIP4 Antibody. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid detection of IgG.PANC-1细胞中的RIP4进行免疫共沉淀，使用抗体Normal Rabbit IgG #2729 (泳道2)或RIP4抗体(泳道3)。泳道1为10%的加入量。Western blot 使用抗体为RIP4抗体。Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678作为二抗用来避免IgG的检测。