| 货号 | 4926S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | RIP Antibody detects endogenous levels of RIP (RIP1) protein. No cross-reactivity was detected with other family members. This antibody also detects a carboxy-terminal fragment of RIP (45 kDa) produced by caspase-8 dependent cleavage. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The RIP (receptor-interacting protein) family of serine-threonine kinases (RIP, RIP2, RIP3 and RIP4) are important regulators of cellular stress that can trigger pro-survival and inflammatory responses through the activation of NF-κB as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and for the recruitment to TNFR1 through interaction with TRADD (2,3). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNFR1 signaling complex via interaction with NEMO leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8 dependent cleavage of the death domain on RIP can trigger the apoptotic activity of RIP (8). RIP-deficient cells show a failure in TNF-mediated NF-κB activation making the cells more sensitive to apoptosis (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
| 参考文献 | 1 . Meylan, E. and Tschopp, J. (2005) Trends Biochem Sci 30, 151-9. 2 . Hsu, H. et al. (1996) Immunity 4, 387-96. 3 . Stanger, B.Z. et al. (1995) Cell 81, 513-23. 4 . Ting, A.T. et al. (1996) EMBO J 15, 6189-96. 5 . Kelliher, M.A. et al. (1998) Immunity 8, 297-303. 6 . Devin, A. et al. (2000) Immunity 12, 419-29. 7 . Zhang, S.Q. et al. (2000) Immunity 12, 301-11. 8 . Lin, Y. et al. (1999) Genes Dev 13, 2514-26. |
Western blot analysis of extracts from HL-60, MOLT-4 and Raji cell lines, using RIP Antibody.Western免疫印迹。用RIP Antibody研究HL-60, MOLT-4 和 Raji 细胞系的细胞提取液。 |
