| 货号 | 2820S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Ring1A Antibody detects endogenous levels of total Ring1A protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ring1A plays a role in Polycomb-group protein function. Polycomb-group (PcG) proteins form two distinct complexes that are critically involved in transcriptional repression of Hox genes during development (1,2). These complexes are (a) EED-EZH2 and (b) the PRC complex, which is comprised of at least Bmi-1 and Ring1A/Ring1B. The EZH2 containing complex is responsible for the methylation of H3K27 and the PRC complex ubiquitylates H2A. EZH2 methylation is required prior to PRC ubiquitylation, and both are essential for Hox gene repression (3). It has recently been shown that PcG proteins silence a group of developmentally important regulator genes, referred to as bivalent genes (4). This regulation may be responsible for the ability of stem cells to self renew or switch to differentiate into multipotent progenitors. Aberrant epigenetic silencing by PcG proteins is also thought to be important in tumorigenesis (5). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Cao, R. et al. (2005) Mol Cell 20, 845-54. 2 . Otte, A.P. and Kwaks, T.H. (2003) Curr Opin Genet Dev 13, 448-54. 3 . Cao, R. et al. (2005) Mol Cell 20, 845-54. 4 . Stock, J.K. et al. (2007) Nat Cell Biol 9, 1428-35. 5 . Sparmann, A. and van Lohuizen, M. (2006) Nat Rev Cancer 6, 846-56. |
Western blot analysis of extracts of MOLT4 annd LNCaP cells using Ring1A Antibody. 使用Ring1A Antibody，免疫印迹(Western blot)分析MOLT4和LNCaP细胞中Ring1A的蛋白水平。 |
