| 货号 | 2579S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Ribosomal Protein S3 Antibody detects endogenous levels of total ribosomal protein S3. |
| 使用方法 | WB(1:1000) IF-IC (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ribosomal protein S3 (rpS3) is a component of the 40S ribosomal subunit and is involved in translation. HSP90 interacts with both the amino-terminus and carboxy-terminus of rpS3, preventing its ubiquitination and degradation and thereby retaining the integrity of the ribosome (1). rpS3 has also been shown to function as an endonuclease during DNA damage repair (2,3). Furthermore, overexpression of rpS3 sensitizes lymphocytic cells to cytokine-induced apoptosis, indicating a third role for rpS3 during apoptosis (4). The functions of rpS3 during DNA damage repair and apoptosis have been mapped to two distinct domains (4). |
| 存放说明 | -20C |
| 计算分子量 | 27 |
| 参考文献 | 1 . Kim, T.S. et al. (2006) Mol Biol Cell 17, 824-33. 2 . Kim, J. et al. (1995) J Biol Chem 270, 13620-9. 3 . Choi, S.H. et al. (2006) FEBS Lett 580, 6755-62. 4 . Jang, C.Y. et al. (2004) FEBS Lett 560, 81-5. |
Confocal immunofluorescent analysis of HeLa cells using Ribosomal Protein S3 Antibody (green). Actin filamens have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Ribosomal Protein S3 Antibody（绿色），共聚焦免疫荧光观察核糖体蛋白S3蛋白在HeLa细胞中定位。Alexa Fluor® 555 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
Western blot analysis of extracts from various cell types using Ribosomal Protein S3 Antibody. 使用Ribosomal Protein S3 Antibody，免疫印迹（Western Blot）分析在不同细胞系中核糖体蛋白S3的蛋白水平。 |
