| 货号 | 2415S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Ribosomal protein L7a Antibody detects endogenous levels of total ribosomal protein L7a. Immunofluorescent analysis using this antibody shows exclusive localization of ribosomal protein L7a to nucleoli during ribosomal biosynthesis. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ribosomal protein L7a is a highly conserved ribosome protein localized to 60S ribosomal subunit (1). The protein has distinct domains that target the newly synthesized polypeptide to nucleus and the nucleoli, the site of ribosome biosynthesis (2). Ribosomal protein L7a can also interact with RNA in vitro through two distinct RNA-binding domains in the protein (3). Taken together, nucleolar localization and the ability to bind RNA suggests that ribosomal protein L7a may act as an important component for ribosome biosynthesis and function. |
| 存放说明 | -20C |
| 计算分子量 | 32 |
| 参考文献 | 1 . De Falco, S. et al. (1993) Gene 126, 227-235. 2 . Russo, G. et al. (1997) J. Biol. Chem. 272, 5229-5235. 3 . Russo, G. et al. (2005) Biochem J. 385, 289-299. |
Confocal immunofluorescent analysis of COS cells using Ribosomal Protein L7a (E109) Antibody (green). Actin filaments have been labeled with Alexa Fluor® phalloidin 555 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Ribosomes are stained by the antibody at their biosynthesis site in nucleoli. 使用Ribosomal Protein L7a (E109) Antibody（绿色），共聚焦免疫荧光观察核糖体蛋白L7a蛋白在COS细胞中定位。Alexa Fluor® phalloidin 555标记微丝蛋白（红色）。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。该抗体在核仁中核糖体生物合成的位置对其进行染色。 | |
Western blot analysis of extracts from various cell lines using Ribosomal Protein L7a (E109) Antibody. 使用Ribosomal Protein L7a (E109) Antibody，免疫印迹（Western Blot）分析在不同细胞系中核糖体蛋白L7a的蛋白水平。 |
