| 货号 | 8959S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Reptin/RuvBL2 Antibody recognizes endogenous levels of total Reptin/RuvBL2 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Reptin/RuvBL2 and Pontin/RuvBL1 are closely related members of the ATPase Associated with diverse cellular Activities (AAA+) super family of proteins that show homology to bacterial RuvB that drives branch migration of Holliday junctions (1). Both proteins can be found together as part of chromatin remodeling and modification complexes such as INO80, TIP60, SRCAP, and Uri1 that are involved in transcription regulation (1,2). Reptin, as part of these complexes, has been implicated in oncogenic transformation driven by c-Myc, β-catenin, and E1A by acting as an essential transcriptional coregulator of target genes (2-7). Reptin also plays a role in modulating cellular responses to hypoxia. Hypoxia induced methylation of Reptin by the methyltransferase G9a leads to its recruitment to hypoxia responsive promoters where it negatively regulates transcription of these genes (8). In addition to transcriptional regulatory roles, Reptin also participates in the telomerase biogenesis processes as part of the telomerase complex and in DNA damage response as part of the TIP60 acetyltransferase complex that stimulates ATM kinase activity necessary for phosphorylation of proteins involved in both checkpoint activation and DNA repair (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Jha, S. and Dutta, A. (2009) Mol Cell 34, 521-33. 2 . Gallant, P. (2007) Trends Cell Biol 17, 187-92. 3 . Huber, O. et al. (2008) Cancer Res 68, 6873-6. 4 . Kim, J.H. et al. (2005) Nature 434, 921-6. 5 . Bauer, A. et al. (2000) EMBO J 19, 6121-30. 6 . Wood, M.A. et al. (2000) Mol Cell 5, 321-30. 7 . Dugan, K.A. et al. (2002) Oncogene 21, 5835-43. 8 . Sun, Y. et al. (2010) Cell Cycle 9, 930-6. 9 . Lee, J.S. et al. (2010) Mol Cell 39, 71-85. 10 . Venteicher, A.S. et al. (2008) Cell 132, 945-57. |
Western blot analysis of extracts from various cell lines using Reptin/RuvBL2 Antibody. 使用Reptin/RuvBL2 Antibody，免疫印迹(Western blot)分析不同细胞中Reptin/RuvBL2的蛋白水平。 |
