| 货号 | 4954S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | RelB Antibody detects endogenous levels of total RelB protein. The antibody does not cross-react with other family members at physiological concentrations. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79. 2 . Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20. 3 . Haskill, S. et al. (1991) Cell 65, 1281-9. 4 . Thompson, J.E. et al. (1995) Cell 80, 573-82. 5 . Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26. 6 . Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. 7 . Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63. 8 . Chen, Z.J. et al. (1996) Cell 84, 853-62. 9 . Senftleben, U. et al. (2001) Science 293, 1495-9. 10 . Coope, H.J. et al. (2002) EMBO J 21, 5375-85. 11 . Xiao, G. et al. (2001) Mol Cell 7, 401-9. |
Western blot analysis of extracts from Raji, A549 and Raw 264.7 cells using RelB Antibody.Western免疫印迹。用RelB Antibody研究Raji, A549 和 Raw 264.7细胞的细胞提取液。 |
