| 货号 | 3667S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | RCC2 Antibody detects endogenous levels of total RCC2 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | RCC2/TD-60 is a member of the RCC1 (regulator of chromosome condensation 1) family of guanine nucleotide exchange factors. RCC2/TD-60 is associated with the chromosome passenger complex (CPC), which also consists of aurora B kinase, borealin, INCENP (inner centromere protein) and survivin. The CPC acts at various stages of mitosis, interacts with microtubules and is required for proper chromosome segregation and cytokinesis. Regulation of aurora B kinase is key in the regulation of the CPC (reviewed in 1,2). In late mitosis, RCC2/TD-60 is required for spindle assembly and recruitment of survivin and aurora B (3). RCC2/TD-60 is also required for aurora B activation in vitro and localization of the CPC to centromeres (4). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . Vader, G. et al. (2006) J Cell Biol 173, 833-7. 2 . Ruchaud, S. et al. (2007) Nat Rev Mol Cell Biol 8, 798-812. 3 . Mollinari, C. et al. (2003) Dev Cell 5, 295-307. 4 . Rosasco-Nitcher, S.E. et al. (2008) Science 319, 469-72. |
Western blot analysis of extracts from HeLa, COS and C2C12 cells using RCC2 Antibody. 使用RCC2 Antibody，免疫印迹（Western Blot）分析HeLa, COS和C2C12细胞中RCC2蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using RCC2 Antibody (green). Actin filaments have been labeled with Pan-Keratin (C11) Mouse mAb #4545 (red). 使用RCC1 (D15H6) Rabbit mAb (绿色)标记，共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。 |
