| 货号 | 3589S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | RCC1 Antibody recognizes endogenous levels of total RCC1 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44. 2 . Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84. 3 . Moore, W. et al. (2002) Curr Biol 12, 1442-7. 4 . Ohtsubo, M. et al. (1989) J Cell Biol 109, 1389-97. 5 . Li, H.Y. and Zheng, Y. (2004) Genes Dev 18, 512-27. 6 . Hutchins, J.R. et al. (2004) Curr Biol 14, 1099-104. 7 . Chen, T. et al. (2007) Nat Cell Biol 9, 596-603. 8 . Wong, C.H. et al. (2009) Nat Cell Biol 11, 36-45. 9 . Horiike, Y. et al. (2009) Mol Biol Rep 36, 717-23. 10 . Dephoure, N. et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7. 11 . Hood, F.E. and Clarke, P.R. (2007) J Cell Sci 120, 3436-45. |
Western blot analysis of extracts from various cell types using RCC1 Antibody. 使用RCC1 Antibody，免疫印迹（Western Blot）分析不同细胞中RCC1蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using RCC1 Rabbit Ab (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). 使用RCC1 Rabbit Ab (绿色)和S6 Ribosomal Protein (54D2) Mouse mAb #2317 (红色)标记，共聚焦免疫荧光分析HeLa细胞。 |
