| 货号 | 3322S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Ras-GRF1 Antibody detects transfected levels of Ras-GRF1. This antibody does not cross-react with Ras-GRF2. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx (1,2). Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity (1,2). Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity (3). Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity (4). It has been reported that Ras-GRF1 also shows GEF activity toward Rac after phosphorylation by the tyrosine kinase Src (5). |
| 存放说明 | -20C |
| 计算分子量 | 155 |
| 参考文献 | 1 . Mattingly, R.R. and Macara, I.G. (1996) Nature 382, 268-72. 2 . Farnsworth, C.L. et al. (1995) Nature 376, 524-7. 3 . Baouz, S. et al. (2001) J. Biol. Chem. 276, 1742-1749. 4 . Mattingly, R.R. (1999) J Biol Chem 274, 37379-84. 5 . Kiyono, M. et al. (2000) J. Biol. Chem. 275, 29788-29793. |
