| 货号 | 3965S |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster/Pig/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras. This antibody may also cross-react with R-Ras and M-Ras. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 21 |
| 参考文献 | 1 . Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54. 2 . Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83. 3 . Buday, L. and Downward, J. (1993) Cell 73, 611-20. 4 . Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31. 5 . Bos, J.L. (1989) Cancer Res 49, 4682-9. 6 . Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31. |
Western blot analysis of extracts from HT-29, PAE, NIH-3T3, AR42J, PC12 and C6 cell lysates,using Ras Antibody.Western blot方法检测细胞提取物：HT-29, PAE, NIH-3T3, AR42J, PC12，C6细胞，使用的抗体是Ras Antibody。 |
