| 货号 | 2554S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | RARα Antibody detects endogenous levels of total RARα protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Retinoids (vitamin A and its active retinoic acid derivatives) are non-steroid hormones that regulate cell proliferation, differentiation and apoptosis. Retinoic acid receptors (RARalpha, -beta and -gamma) and retinoid X receptors (RXRalpha, -beta and -gamma) are nuclear receptors that function as RAR-RXR heterodimers or RXR homodimers (1-2). In response to retinoid binding, these dimers control gene expression by binding to specific retinoic acid response elements, by recruiting cofactors and the transcriptional machinery, and by indirectly regulating chromatin structure. Finally, ligand binding and phosphorylation of RARalpha by JNK at Thr181, Ser445 and Ser461 controls the stability of RAR-RXR through the ubiquitin-proteasome pathway (3-4). At least four distinct genetic lesions affect RARalpha and result in acute promyelocytic leukemia (APL). The t(15;17) translocation that results in the PML-RARalpha fusion protein is responsible for more than 99% of APL cases, and the fusion protein inhibits PML-dependent apoptotic pathways in a dominant negative fashion. In addition PML-RARalpha inhibits transcription of retinoic acid target genes by recruiting co-repressors, attenuating myeloid differentiation (5-6).维甲酸（维生素A及其活性维甲酸衍生物）是非类固醇类激素，可以调节细胞增殖、分化和凋亡。维甲酸受体（RAR-α，-β和-γ）和维甲酸X受体（RXR-α，-β和-γ）都是核受体，组成RAR-RXR异二聚体或RXR二聚体起作用（1-2）。这些二聚体通过与特异性维甲酸应答元件结合、招募辅酶因子、转录机制和间接调节染色质结构来控制基因表达，响应维甲酸结合。最后，JNK配体结合并磷酸化RARα苏氨酸（181位）、丝氨酸（445和461位），通过泛素-蛋白酶体通路控制RAR-RXR的稳定性（3-4）。至少四个不同的遗传病变影响RARα并导致急性早幼粒细胞白血病（APL）。t（15;17）的易位会导致PML- RARα融合蛋白负责99％以上APL患者，融合蛋白以显性抑制方式抑制PML-依赖性的凋亡通路。此外PML-RARα通过招募辅阻遏物、衰减髓样分化抑制维甲酸靶基因转录（5-6）。 |
| 存放说明 | -20C |
| 计算分子量 | 55 |
| 参考文献 | 1 . Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9. 2 . Mangelsdorf, D.J. and Evans, R.M. (1995) Cell 83, 841-850. 3 . Bastien, J. and Rochette-Egly, C. (2004) Gene 328, 1-16. 4 . Srinivas, H. et al. (2005) Mol. Cell. Biol. 25, 1054-1069. 5 . de The, H. et al. (1990) Nature 347, 558-561. 6 . Slack, J.L. and Rusiniak, M.E. (2000) Ann. Hematol. 79, 227-238. |
Western blot analysis of extracts from NIH/3T3 and C6 cells, using RARα Antibody.Western blot方法检测NIH/3T3 细胞和C6细胞提取物，使用的抗体为RARα Antibody. |
