| 货号 | 93706S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | RanBP3 Antibody recognizes endogenous levels of total RanBP3 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1). It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3). In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex. In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4). In some cases, as with β-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6). RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway. This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70, 90 |
| 参考文献 | 1 . Mueller, L. et al. (1998) FEBS Lett 427, 330-6. 2 . Lindsay, M.E. et al. (2001) J Cell Biol 153, 1391-402. 3 . Englmeier, L. et al. (2001) EMBO Rep 2, 926-32. 4 . Nemergut, M.E. et al. (2002) J Biol Chem 277, 17385-8. 5 . Hendriksen, J. et al. (2005) J Cell Biol 171, 785-97. 6 . Dai, F. et al. (2009) Dev Cell 16, 345-57. 7 . Yoon, S.O. et al. (2008) Mol Cell 29, 362-75. 8 . von Knethen, A. et al. (2010) J Cell Sci 123, 192-201. |
Western blot analysis of extracts from U-118 MG, NIH/3T3, and Neuro-2a cells using RanBP3 Antibody. | |
Immunoprecipitation of RanBP3 protein from U-118 MG cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is RanBP3 Antibody. Western blot analysis was performed using RanBP3 Antibody. |
