| 货号 | 4462S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Ran Antibody detects endogenous levels of total Ran protein. The antibody does not cross-react with other small GTPases of the Ras family. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3). |
| 存放说明 | -20C |
| 计算分子量 | 24 |
| 参考文献 | 1 . Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44. 2 . Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84. 3 . Yamaguchi, R. and Newport, J. (2003) Cell 113, 115-125. |
Western blot analysis of extracts from HeLa, NIH/3T3, C6, COS, BAEC, and CHO cells, using Ran Antibody. 使用Ran Antibody，免疫印迹（Western Blot）分析HeLa、NIH/3T3、C6、COS、BAEC和CHO细胞中Ran蛋白水平。 |
