| 货号 | 3630S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | RalBP1 (I33) Antibody detects endogenous levels of total RalBP1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The RalA binding protein 1 (RalBP1 or RLIP76) was originally identified as a GTP-RalA associated protein that acted as a downstream RalA effector in regulating Ral-Ras signaling (1). RalBP1 interacts with RalA and the endocytosis protein REPS2 (POB1) through its carboxy-terminal Ral binding domain. RalBP1 has an intrinsic GTPase activating function and interacts with Cdc42 through its centrally located Rho-GAP domain (1-3). A protein complex containing RalBP1/POB1/RalA regulates endocytosis of membrane receptors (4). RalBP1 also functions as a non-ABC transporter that catalyzes the ATP-dependent transport of numerous xenobiotics, including glutathione conjugates and some chemotherapeutic agents. RalBP1 transporter activity may play an important role in detoxification, drug resistance and the stress response (5-7). Increased expression of RalBP1 protein is associated with some forms of cancer and regression of cancer xenografts results from RalBP1 inhibition (8,9). Evidence to date suggests that RalBP1 may be a promising therapeutic target for cancer therapy. |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Cantor, S.B. et al. (1995) Mol Cell Biol 15, 4578-84. 2 . Matsubara, K. et al. (1997) FEBS Lett 410, 169-74. 3 . Ikeda, M. et al. (1998) J Biol Chem 273, 814-21. 4 . Nakashima, S. et al. (1999) EMBO J 18, 3629-42. 5 . Awasthi, Y.C. et al. (2007) Curr Drug Metab 8, 315-23. 6 . Awasthi, S. et al. (2002) Drug Metab Dispos 30, 1300-10. 7 . Awasthi, S. et al. (2008) Clin Cancer Res 14, 4372-7. 8 . Hudson, M.E. et al. (2007) Proc Natl Acad Sci USA 104, 17494-9. 9 . Singhal, S.S. et al. (2007) Cancer Res 67, 4382-9. |
Western blot analysis of extracts from MCF-7, C6 and COS cells using RalBP1 (I33) Antibody. 使用RalBP1 (I33) Antibody，免疫印迹（Western Blot）分析MCF-7、C6和COS细胞中RalBP1蛋白水平。 |
