| 货号 | 3360S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | RagC Antibody detects endogenous levels of total RagC protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | mTORC1 kinase complex is a critical component in regulating cell growth (1,2). Its activity is modulated by energy levels, growth factors and amino acids (3,4). Recent studies found that RagA, RagB, RagC and RagD, the four related GTPases, interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Sancak, Y. et al. (2008) Science 320, 1496-501. 2 . Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 3 . Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 4 . Wullschleger, S. et al. (2006) Cell 124, 471-84. |
Western blot analysis of extracts from various cell types using RagC Antibody. Western blot 检测分析各种细胞提取物，使用的抗体是RagC Antibody。 | |
Confocal immunofluorescent analysis of HeLa cells using RagC Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测Hela细胞，使用的抗体是RagC Antibody（绿色）。微丝用DY-554 phalloidin标记（红色）。蓝色伪彩是 DRAQ5® #4084 (fluorescent DNA dye)。 |
