| 货号 | 3427S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Rad50 antibody detects endogenous levels of total Rad50 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and Nbs1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2).DNA修复蛋白Rad50是染色体结构维持家族(SMC)的一员，在细胞周期检验点信号和DNA损伤的双链断裂修复应答过程中发挥重要作用(1-4)。Rad50和Mre11及Nbs1组成一个蛋白复合体，当DNA损伤时被激活(3)。在正常的人类细胞中，MRN复合物能够拴住线性DNA分子，在DNA末端之间提供一个弹性链接(1)。细胞中的基因突变能够影响MRN复合物中的蛋白导致基因组不稳定和癌症的发生(2)。 |
| 存放说明 | -20C |
| 计算分子量 | 153 |
| 参考文献 | 1 . de Jager, M. et al. (2001) Mol. Cell 8, 1129-35. 2 . Elledge, S.J. (1996) Science 274, 1664-1672. 3 . Lee, J.H. and Lim, D.S. (2006) FEBS J. 273, 1630-36. 4 . Teng, S.C. et al. (2006) J Mol Histol 37, 293-9. 5 . Gatei, M. et al. (2011) J Biol Chem 286, 31542-56. |
Western blot analysis of extracts from Jurkat and K562 cells, using RAD50 Antibody.Western blot 方法检测Jurkat和K562细胞，使用的抗体为 RAD50 Antibody。 |
